The carryover is defined as the difference between the absorbance of (4) Sample 1 (immediately after switching from water to the sample) and the absorbance of (6) Sample 3, divided by the absorbance measured for (6) Sample 3. The sequence of measurements was: (1) Water 1, (2) Water 2, (3) Water 3, (4) Sample 1, (5) Sample 2, and (6) Sample 3. The absorbance at 350 nm was measured three times each for water (blank) and the sample.
The sample was an aqueous solution of potassium dichromate prepared to approximately 0.5 Abs. Table 3 shows a list of the sipper setting conditions. We followed the method described in the instruction manual to test the carryover of a UV-1800 with Syringe Sipper N and a square flow cell (micro) with 1.0 mL standard required sample volume.
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